Daniel Winston Bellott

Supplementary Information for:

A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes

Michelle A Carmell, Gregoriy A Dokshin, Helen Skaletsky, Yueh-Chiang Hu, Josien C van Wolfswinkel, Kyomi J Igarashi, Daniel W Bellott, Michael Nefedov, Peter W Reddien, George C Enders, Vladimir N Uversky, Craig C Mello, David C Page

eLife 5, e19993 (2016)

Figures:

Figure 1 Source data 1: (XLS)

Mass spectrometry data

Figure 1 Figure supplement 1: (JPG) (Figure legend)

Characterization of mouse Gcna transcript and protein

Figure 1 Figure supplement 2: (JPG) (Figure legend)

Generation of Gcna-targeted ES cells and mice

Figure 2 Source data 1: (XLS)

Charge/hydropathy analysis

Figure 3 Figure supplement 1: (JPG) (Figure legend)

Loss of structured domains in the murine lineage

Figure 3 Figure supplement 2: (JPG) (Figure legend)

Alignment of vertebrate GCNA proteins

Figure 4 Source data 1: (XLS)

List of GCNA family members

Figure 4 Source data 2: (XLS)

Reproductive expression of GCNA family members

Figure 4 Figure supplement 1: (JPG) (Figure legend)

Alignments and probabilistic hidden Markov models (HMMs) of structured domains found in GCNA family members

Figure 4 Figure supplement 1 - source data 1: (DOC)

Hidden Markov models used to discover GCNA orthologs

Figure 4 Figure supplement 2: (JPG) (Figure legend)

GCNA proteins in higher plants comprise the YABBY family

Figure 4 Figure supplement 3: (JPG) (Figure legend)

GCNA and YABBY HMG boxes are more closely related to each other than they are to any other type of HMG box, either within plants or across eukarya

Figure 5 Figure supplement 1: (JPG) (Figure legend)

Enrichment of Gcna expression in reproductive tissues of vertebrates

Figure 6 Figure supplement 1: (JPG) (Figure legend)

C. elegans gcna-1 alleles

Figure 8 Figure supplement 1: (JPG) (Figure legend)

GCNA, Spartan, and Wss1 form a subgroup within the larger Peptidase Clan MA

Files:

Source Code 1: (DOC) (TXT)

Perl script for isoelectric point calculation