Generation of Gcna-targeted ES cells and mice.

(A) Mouse Gcna gene targeting strategy. Purple triangles are LoxP sites and red ovals are FRT recombination sites. Coding portions of exons are dark gray while UTRs are light gray. (B) Southern blot using probe indicated by asterisk after digesting genomic DNA with NheI. The probe and the 5’ NheI site are both outside of the homology arms.