C. elegans gcna-1 alleles.

(A) Diagrams of wildtype (N2), ne4334 (2-bp deletion), and ne4356 (1748-bp deletion) alleles. (B) Sequencing traces confirming the 2-bp deletion in the ne4334 allele. (C) PCR genotyping of the ne4356 allele confirming the large deletion. F3/R1 primers bind outside of the deletion, while the binding site of primer F1 is within the deleted region.